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ISOLATION AND CULTURE OF TOMATO MESOPHYLL PROTOPLASTS
Author(s) -
De Wit Pierre J. G. M.
Publication year - 1976
Publication title -
acta botanica neerlandica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.871
H-Index - 87
eISSN - 1438-8677
pISSN - 0044-5983
DOI - 10.1111/j.1438-8677.1976.tb01082.x
Subject(s) - protoplast , isolation (microbiology) , botany , incubation , horticulture , cell wall , division (mathematics) , biology , salt (chemistry) , chemistry , microbiology and biotechnology , biochemistry , mathematics , arithmetic
SUMMARY Viable tomato mesophyll protoplasts were isolated by using the non‐phytotoxic macerating enzyme PATE instead of the generally used Macerozyme. To obtain the osmotic conditions required during isolation, salt solutions, containing 2.5% (w/v) KCl and 1% (w/v) MgSO 4 · 7H 2 O, were used. From studies in different culture media it appeared that, under optimum conditions, cells regenerated a wall and divided three to four times. After about three weeks of incubation cell division stopped and cells started to degenerate.