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STUDIES ON PHLOEM EXUDATION FROM YUCCA FLACCIDA HAW. XIII. EVIDENCE FOR THE OCCURRENCE OF A CYTOKININ NUCLEOTIDE IN THE EXUDATE
Author(s) -
Vonk C. R.
Publication year - 1974
Publication title -
acta botanica neerlandica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.871
H-Index - 87
eISSN - 1438-8677
pISSN - 0044-5983
DOI - 10.1111/j.1438-8677.1974.tb00968.x
Subject(s) - cytokinin , exudate , zeatin , phloem , nucleotide , biochemistry , botany , biology , chemistry , auxin , gene
SUMMARY Phloem exudate of Yucca was analysed for cytokinin activity. After partition in a water‐ and butanol‐1 fraction, almost no cytokinin activity was found in the butanol phase (cytokinin base and nucleosides). Application of alkaline phosphatase to the water layer gave rise to a strong positive cytokinin response with the soy‐bean callus test, an amount of approximately 520 μg zeatin equivalent per litre being found in the exudate after treatment with alkaline phosphatase. The results indicate that a cytokinin, presumably zeatin, is translocated as a nucleotide.