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PHOTO‐OXIDATIVE DAMAGE TO TRIPHENYL‐TETRAZOLIUMCHLORIDE (TTC) REDUCING CAPACITY OF CUCUMIS LEAF DISCS DURING CHILLING
Author(s) -
Van Hasselt Ph. R.
Publication year - 1973
Publication title -
acta botanica neerlandica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.871
H-Index - 87
eISSN - 1438-8677
pISSN - 0044-5983
DOI - 10.1111/j.1438-8677.1973.tb00876.x
Subject(s) - cucumis , carotenoid , pigment , oxygen , chemistry , oxidative damage , chlorophyll , oxidative phosphorylation , photosynthesis , horticulture , botany , biophysics , food science , antioxidant , biochemistry , biology , organic chemistry
SUMMARY The effect of light, during pretreatment at 1 °C, on the capacity of Cucumis leaf discs to reduce TTC 1 was studied. Light decreases the capacity of the cells to reduce TTC by a photo‐oxidative process. The kinetics show a lag phase followed by a rapid decrease. 6% oxygen as compared to 100% oxygen lengthens the lag phase but does not alter the rate of damage during the rapid phase. DCMU 2 has an inhibitive effect. A crude action spectrum of the decrease in TTC reduction shows that the blue and the red region of the spectrum are most effective, while the green and the far red region are relatively ineffective. It is suggested that chlorophyll as well as the carotenoids absorb the light energy which causes the harmful effect on the mechanism of TTC reduction.