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INCORPORATION OF UDP‐GALACTOSE‐ 14 C BY SPINACH CHLOROPLASTS
Author(s) -
Helmsing P. J.,
Barendse G. W. M.
Publication year - 1970
Publication title -
acta botanica neerlandica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.871
H-Index - 87
eISSN - 1438-8677
pISSN - 0044-5983
DOI - 10.1111/j.1438-8677.1970.tb00686.x
Subject(s) - chloroplast , galactose , incubation , chemistry , spinach , biochemistry , chromatography , gene
SUMMARY The incorporation of UDP‐galactose‐ 14 C into lipids of spinach chloroplasts as affected by different experimental conditions was studied. It was found that the use of a pyrophosphate buffer and the addition of albumine during isolation and incubation of chloroplasts enhance the incorporation of UDP‐galactose‐ 14 C compared with other buffers or no addition of albumine. Furthermore, the ability of chloroplasts to incorporate UDP‐galactose decreased with the age of the chloroplasts. The rate of UDP‐galactose incorporation into lipids of the chloroplasts appears to depend on the condition of the chloroplasts.