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THE INFLUENCE OF THE EFFLUENT AND OF THE TEMPERATURE ON THE SEPARATION OF INDOLEACETIC ACID OXIDASE OF PEA ROOTS ON SEPHADEX COLUMNS
Author(s) -
Janssen M. G. H.
Publication year - 1970
Publication title -
acta botanica neerlandica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.871
H-Index - 87
eISSN - 1438-8677
pISSN - 0044-5983
DOI - 10.1111/j.1438-8677.1970.tb00664.x
Subject(s) - sephadex , adsorption , effluent , chemistry , chromatography , elution , ionic strength , enzyme , oxidase test , biochemistry , organic chemistry , environmental engineering , aqueous solution , engineering
SUMMARY The low molecular IAA‐oxidase of pea roots, reported previously (Janssen 1969b), is formed from the larger units during the chromatographic procedure. This formation is enhanced both by increasing temperatures and by increasing ionic strength of the effluent. It is also demonstrated that the low molecular IAA‐oxidase can adsorb to the Sephadex. Once adsorbed the enzyme molecules can be eluted with a NaCl solution.