Open AccessDanshen protects kidney grafts from ischemia/reperfusion injury after experimental transplantation
Author(s) -
Guan Xiaohai,
DeiAnane Genevieve,
Bruns Helge,
Chen Jing,
Nickkholgh Arash,
Liang Rui,
Gross MarieLuise,
Kern Michael,
Ludwig Jochen,
Büchler Markus W.,
Schemmer Peter
Publication year - 2009
Publication title -
transplant international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.998
H-Index - 82
eISSN - 1432-2277
pISSN - 0934-0874
DOI - 10.1111/j.1432-2277.2008.00770.x
Subject(s) - medicine , creatinine , lactate dehydrogenase , transplantation , kidney , blood urea nitrogen , aspartate transaminase , reperfusion injury , renal function , alanine transaminase , ischemia , nitric oxide synthase , acute tubular necrosis , endocrinology , nitric oxide , pharmacology , biochemistry , chemistry , alkaline phosphatase , enzyme
Summary Danshen (DS) is used for treatment of various ischemic events in the traditional Chinese medicine. Hence, this study was designed to investigate its effect on ischemia/reperfusion injury (IRI) after experimental kidney transplantation (eKTx). Nephrectomized Sprague–Dawley rats underwent eKTx. Some animals were infused with 1.5 ml DS 10 min before surgery. Kidney grafts were transplanted after cold storage for 20 h in Histidine–Tryptophane–Ketoglutarate solution. After reperfusion blood samples were collected for blood urinary nitrogen (BUN), creatinine, lactate dehydrogenase (LDH), and alanine transaminase. Further, tissue was assessed for morphologic and pathophysiologic changes. Donor preconditioning with DS (DS‐d) significantly decreased BUN, creatinine, LDH, and aspartate aminotransferase to 65–97% of controls while preconditioning of the recipient (DS‐r) decreased values to 58–82% ( P < 0.05). Tubular damage and caspase‐3 decreased significantly in both DS‐d and DS‐r (DS‐d: 96% and 67%, DS‐r: 83% and 75% of controls) while heat shock protein 72 and superoxide dismutase increased significantly (DS‐d: 143% and 173%, DS‐r: 166% and 194% of controls). Further, inducible nitric oxide synthase and tumor necrosis factor‐α decreased (DS‐d: 84% and 61%, DS‐r: 79% and 67% of controls) after DS. Preconditioning of both donors and recipients with DS significantly reduces IRI and thus improves graft function after eKTx.