Open AccessA Role for galectin‐3 in renal tissue damage triggered by ischemia and reperfusion injury
Author(s) -
Fernandes Bertocchi Ana Paula,
Campanhole Gabriela,
Wang Pamella Huey Mei,
Gonçalves Giselle Martins,
Damião Márcio José,
Cenedeze Marcos Antônio,
Beraldo Felipe Caetano,
De Paula Antunes Teixeira Vicente,
Dos Reis Marlene Antônia,
Mazzali Marilda,
PachecoSilva Alvaro,
Câmara Niels Olsen Saraiva
Publication year - 2008
Publication title -
transplant international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.998
H-Index - 82
eISSN - 1432-2277
pISSN - 0934-0874
DOI - 10.1111/j.1432-2277.2008.00705.x
Subject(s) - medicine , reactive oxygen species , inflammation , chemokine , galectin 3 , oxidative stress , proinflammatory cytokine , kidney , immunology , reperfusion injury , knockout mouse , ischemia , chemistry , biochemistry , receptor
Summary Ischemic‐reperfusion injury (IRI) triggers an inflammatory response involving neutrophils/macrophages, lymphocytes and endothelial cells. Galectin‐3 is a multi‐functional lectin with a broad range of action such as promotion of neutrophil adhesion, induction of oxidative stress, mastocyte migration and degranulation, and production of pro‐inflammatory cytokines. The aim of this study was evaluate the role of galectin‐3 in the inflammation triggered by IRI. Galectin‐3 knockout (KO) and wild type (wt) mice were subjected to 45 min of renal pedicle occlusion. Blood and kidney samples were collected at 6, 24, 48 and 120 h. Blood urea was analyzed enzymatically, while MCP‐1, IL‐6 and IL‐1β were studied by real‐time PCR. Reactive oxygen species (ROS) was investigated by flow cytometry. Morphometric analyses were performed at 6, 24, 48 and 120 h after reperfusion. Urea peaked at 24 h, being significantly lower in knockout animals (wt = 264.4 ± 85.21 mg/dl vs. gal‐3 KO = 123.74 ± 29.64 mg/dl, P = 0.001). Galectin‐3 knockout animals presented less acute tubular necrosis and a more prominent tubular regeneration when compared with controls concurrently with lower expression of MCP‐1, IL‐6, IL‐1β, less macrophage infiltration and lower ROS production at early time points. Galectin‐3 seems to play a role in renal IRI involving the secretion of macrophage‐related chemokine, pro‐inflammatory cytokines and ROS production.