z-logo
open-access-imgOpen Access
In vivo magnetic resonance imaging of vascularization in islet transplantation
Author(s) -
Hathout Eba,
Sowers Lawrence,
Wang Rong,
Tan Annie,
Mace John,
Peverini Ricardo,
Chinnock Richard,
Obenaus Andre
Publication year - 2007
Publication title -
transplant international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.998
H-Index - 82
eISSN - 1432-2277
pISSN - 0934-0874
DOI - 10.1111/j.1432-2277.2007.00550.x
Subject(s) - islet , medicine , transplantation , in vivo , magnetic resonance imaging , neovascularization , gadolinium , contrast (vision) , microsphere , ex vivo , preclinical imaging , pathology , nuclear medicine , angiogenesis , radiology , diabetes mellitus , endocrinology , chemistry , biology , microbiology and biotechnology , organic chemistry , artificial intelligence , chemical engineering , computer science , engineering
Summary To evaluate changes in neovascularization of transplanted islets in vivo , dynamic contrast (gadolinium) enhanced magnetic resonance imaging (MRI) was used. Both iron (Feridex)‐labeled and unlabeled syngeneic murine subcapsular islet grafts were studied. Differences in dynamic contrast enhancement of islet grafts were quantified after gadolinium injection at post‐transplant days 3 and 14. Normalized contrast concentrations at day 14 in transplanted islets were increased relative with that on day 3. Time to peak contrast enhancement was faster by 12 min at day 14 compared to day 3 islets (while kidney and muscle peak times remained the same). Areas under the curve for contrast concentration versus time plots were larger in 14‐day relative to 3‐day islet grafts. In conclusion, noninvasive assessment of neovascularization is achievable. In vivo dynamic contrast‐enhanced MRI can be used to detect and quantify changes in vascularization following islet transplantation. This technique may be useful in developing pro‐angiogenic strategies to improve the transplantation outcome in experimental and clinical settings.

The content you want is available to Zendy users.

Already have an account? Click here to sign in.
Having issues? You can contact us here