Open AccessStatic normothermic preservation of renal allografts using a novel nonphosphate buffered preservation solution
Author(s) -
Kay Mark D.,
Hosgood Sarah A.,
Harper Simon J. F.,
Bagul Atul,
Waller Helen L.,
Rees Douglas,
Nicholson Michael L.
Publication year - 2007
Publication title -
transplant international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.998
H-Index - 82
eISSN - 1432-2277
pISSN - 0934-0874
DOI - 10.1111/j.1432-2277.2006.00390.x
Subject(s) - medicine , viaspan , renal function , machine perfusion , perfusion , urology , creatinine , cold storage , kidney , transplantation , vascular resistance , renal blood flow , blood pressure , biology , horticulture , liver transplantation
Summary The aim of this study was to assess the viability and function of renal allografts under normothermic conditions using a novel nonphosphate buffered preservation solution AQIX®RS‐I. Porcine kidneys were flushed at 30 °C with AQIX®RS‐I at 100 mmHg pressure after 5–10 min warm ischaemic time and stored statically at either 4 °C or 30 °C for 2 h ( n = 6 per group). Assessment of renal function by physiological and biochemical parameters was performed by perfusing the organs with autologous blood at 37 °C, with an initial circulating serum creatinine concentration of 1000 μ mol/l on an isolated organ perfusion system for 6 h. Although the hypothermic group demonstrated overall superior renal function, the normothermic stored kidneys displayed a statistically comparable acid‐base balance (7.37 ± 0.15 vs. 7.3 ± 0.09, P = 0.24). Furthermore, renal function was still evident after 6 h perfusion with increasing oxygen consumption, renal blood flow and reduced renal vascular resistance. The effectiveness and versatility of AQIX ® RS‐I as a preservation solution under both normothermic and hypothermic conditions has been demonstrated. Renal viability was maintained after 2 h static normothermic storage. This study provides a foundation for further analysis utilizing normothermic preservation.