Open AccessIntravenous transplantation of mesenchymal stem cells improves cardiac performance after acute myocardial ischemia in female rats
Author(s) -
Jiang Wenhui,
Ma Aiqun,
Wang Tingzhong,
Han Ke,
Liu Yu,
Zhang Yanmin,
Zhao Xiaoge,
Dong Anping,
Du Yuan,
Huang Xin,
Wang Jun,
Lei Xinjun,
Zheng Xiaopu
Publication year - 2006
Publication title -
transplant international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.998
H-Index - 82
eISSN - 1432-2277
pISSN - 0934-0874
DOI - 10.1111/j.1432-2277.2006.00307.x
Subject(s) - mesenchymal stem cell , medicine , transplantation , homing (biology) , preload , ligation , myocyte , stem cell , cardiology , pathology , andrology , hemodynamics , biology , microbiology and biotechnology , ecology
Summary Mesenchymal stem cells (MSCs) are potential sources of cells for tissue repairing. However, little information is available regarding the therapeutic potency of intravenously transplanted MSCs for myocardial ischemia (MI). In the present study, MSCs were isolated from bone marrow of male rats and expanded in vitro . Three hours after ligation of left anterior descending artery, the transplanted group received an infusion of MSCs through the tail vein. At the same time, a coronary‐ligated control group was injected with culture medium. Homing of MSCs to the heart was assessed by expression of the Y chromosome sry gene using fluorescent in situ hybridization (FISH). At 1 week or 8 weeks after transplantation, sry positive cells were present in cardiac tissue in the transplanted group, but not in the hearts of control group. Cardiomyocytes, smooth muscle cells, and endothelial cells that bore sry gene were identified in transplanted group at 8 weeks after transplantation. Ultrastructural observation revealed that a large number of capillary and some immature myocytes were found to survive in the ischemia region. MSCs transplantation also decreased LVEDP pressure and −dP/d t , but increased LVSP and +dP/d t . The cardiac infarct size was significantly smaller in transplanted group than in control group. Our data suggest that intravenously transplanted MSCs improve cardiac performance and promote the regeneration of blood vessels and cardiomyocytes.