Open AccessInfluence of nitric oxide on microcirculation in pancreatic ischemia/reperfusion injury: an intravital microscopic study
Author(s) -
Obermaier R.,
Dobschuetz E.,
Muhs O.,
Keck T.,
Drognitz O.,
Jonas L.,
Schareck W.,
Hopt U. T.,
Benz S.
Publication year - 2004
Publication title -
transplant international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.998
H-Index - 82
eISSN - 1432-2277
pISSN - 0934-0874
DOI - 10.1111/j.1432-2277.2004.tb00430.x
Subject(s) - nitric oxide , sodium nitroprusside , microcirculation , medicine , ischemia , reperfusion injury , intravital microscopy , anesthesia , saline , pharmacology
Recently, protective effects of nitric oxide donors in pancreatic ischemia/reperfusion (IRI) injury have been described. Their role in post‐ischemic microcirculation was previously not investigated. Ischemia reperfusion was induced in an isolated pancreatic tail segment in situ. Animals were randomized to four experimental groups ( n = 7 animals/group), the control group (CO) received saline as placebo. Treatment groups received either sodium nitroprusside (SN) 5 min before until 2 h after reperfusion, l ‐arginine (LA) 30 min before reperfusion until 2 h after reperfusion or sodium nitroprusside and l ‐arginine (SNLA) together. After induction of ischemia (2 h) post‐ischemic microcirculation was observed for 2 h by intravital‐fluorescence microscopy. Functional‐capillary density (FCD), leukocyte adherence in post‐capillary venules (LAV) and histological damage were analysed. After reperfusion FCD decreased in all groups ( p <0.05). FCD was significantly restored in all groups with administration of nitric oxide donors after reperfusion ( p <0.05) as compared to CO without significant difference between the individual nitric oxide donor groups. Leukocyte adherence was significantly increased 1 h and 2 h after reperfusion ( p <0.001) as compared to baseline, which was lower in all nitric oxide donor groups. Histological damage in the pancreatic tail‐segment was significantly reduced in nitric oxide donor groups ( p <0.01). Administration of nitric oxide donors might be useful in ischemia‐reperfusion injury of the pancreas by its protective effect on microcirculation and inflammatory reaction.