Open AccessUpregulation of intragraft interleukin‐10 by infusion of granulocyte colony‐stimulating factor‐mobilized donor leukocytes
Author(s) -
Egi Hiroyki,
Hayamizu Keisuke,
Ohmori Ichiro,
Kitayama Teruhiko,
Asahara Toshimasa
Publication year - 2002
Publication title -
transplant international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.998
H-Index - 82
eISSN - 1432-2277
pISSN - 0934-0874
DOI - 10.1111/j.1432-2277.2002.tb00203.x
Subject(s) - medicine , granulocyte colony stimulating factor , granulocyte , downregulation and upregulation , immunology , interleukin , interleukin 10 , cytokine , chemotherapy , biology , biochemistry , gene
We examined the effects of granulocyte colony‐stimulating factor (G‐CSF)‐mobilized donor leukocyte infusion (G‐DLI) on facilitation of allograft survival using heart transplantation from DA to Lewis rats that were transiently treated with tacrolimus (2 mg/kg i.m. on day 0). Other DA rats were given G‐CSF (250 μ/kg/day s.c. from days −5 to 0), and isolated leukocytes were infused into Lewis recipients after surgery. Cytokine mRNA levels were quantified by reverse transcription and real‐time polymerase chain reaction. After G‐CSF treatment, leukocytes in circulation increased by 7.6 times and secreted in‐vitro 6.0‐times‐higher levels of IL‐10 after lipopolysaccharide stimulation than did untreated leukocytes. G‐DLI facilitated graft survival dose‐dependently. Significant IL‐10 mRNA upregulation was detected in grafts 24 h after surgery but not in the recipient's heart, spleen, or liver. On day 6, IFN‐γ and IL‐2 mRNA levels were approximately half those of the control levels. Allograft‐restricted IL‐10 upregulation followed by type‐1 cytokine downregulation can be achieved by the use of G‐DLI.