Open AccessImpact of current cryopreservation procedures on mechanical and functional properties of human aortic homografts
Author(s) -
Langerak S. E.,
Groenink M.,
Wall E. E.,
Wassenaar C.,
Vanbavel E.,
Spaan J. A. E.,
Baal M. C.
Publication year - 2001
Publication title -
transplant international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.998
H-Index - 82
eISSN - 1432-2277
pISSN - 0934-0874
DOI - 10.1111/j.1432-2277.2001.tb00053.x
Subject(s) - cryopreservation , contractility , elastin , thoracic aorta , endothelium , medicine , andrology , aorta , aortic valve , biomedical engineering , anatomy , cardiology , pathology , microbiology and biotechnology , biology , embryo
We evaluated the impact of standard cryopreservation on mechanical and functional properties of human aortic homografts. From 14 human heart‐valve donors, the thoracic descending aorta was obtained. Effects of cryopreservation on mechanical (elastic properties and breaking stress) and smooth muscle cell (SMC) and endothelium function were tested. Cryopreservation (cryo) did not significantly affect Young's modulus of elastin (fresh: 3.1 ± 1.0, cryo: 2.7 ± 0.9 ± 10 5 Nm ‐2 ), collagen recruitment pressure (fresh: 1.1 ± 0.3, cryo: 1.1 ± 0.4 ± 10 4 Nm ‐2 ), distensibility (fresh: 3.8 ± 1.8, cryo: 3.6 ± 1.6 ± 10 5 N ‐1 m 2 ), or breaking stress (fresh: 2.4 ± 1.0, cryo: 2.2 ± 1.0 ± 10 6 Nm ‐2 ). Following explantation, no endothelium‐dependent relaxation was found. SMC function and endothelium‐independent relaxation were mainly intact after explantation but significantly decreased after cryopreservation. Aortic mechanical properties are not influenced by cryopreservation. Following explantation, almost no endothelial cell function is present, and SMC contractility is strongly affected after cryopreservation.