Open AccessCD4+ and CD8+ T cell subset distribution in the blood of small‐bowel‐grafted rats: modification during graft‐versus‐host disease
Author(s) -
Marni A.,
Forti D.,
Fulgenzi A.,
Corsi M.,
Cazzulani A.,
Ferrero M.E.
Publication year - 1998
Publication title -
transplant international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.998
H-Index - 82
eISSN - 1432-2277
pISSN - 0934-0874
DOI - 10.1111/j.1432-2277.1998.tb01183.x
Subject(s) - medicine , cd8 , graft versus host disease , gastroenterology , transplantation , monoclonal antibody , immunology , peripheral blood , antibody , immune system
We studied the modifications of blood T cell distribution following small‐bowel allografting in rats under different experimental conditions. Group 1: ACI (RT1 a ) rats were used as small‐bowel donors for ACI × Wistar (RT1 y ) F 1 , hybrid rats (WAF 1 ) in which graft‐versus‐ host disease (GVHD) developed. Group 2: WAF 1 rats were used as small bowel donors to ACI rats which developed rejection. Group 3: WAF 1 rats received small bowel from ACI rats hyperimmunized for 10 days (by grafting them with WAF 1 skin) and GVHD developed. Group 4: Wistar rats received small bowel from ACI rats hyperimmunized for 10 days (by Wistar skin) and bidirectional GVHD and rejection were assured. A second set of the same groups which were continuously administered with cyclosporin (15 mg/kg per day s.c. for 15 consecutive days) was also studied. Recipient peripheral blood lymphocytes, obtained at 7 and 15 days following small‐bowel transplantation, were stained with monoclonal antibodies anti‐rat CD4 and CD8 and then analyzed in an automated flow cytometer. A significant major reduction of CD4 + /CD8 + T cell ratios was shown in rats that developed simultaneous GVHD and rejection with respect to ungrafted rats.