Open AccessThe effect of cyclosporine on endothelin levels after orthotopic liver transplantation in rats
Author(s) -
Shiraishi M.,
Kusano T.,
Hara J.,
Hiroyasu S.,
Miyaguni T.,
Muto Y.
Publication year - 1997
Publication title -
transplant international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.998
H-Index - 82
eISSN - 1432-2277
pISSN - 0934-0874
DOI - 10.1111/j.1432-2277.1997.tb00687.x
Subject(s) - medicine , radioimmunoassay , endothelin 1 , endocrinology , endothelin receptor , kidney , liver transplantation , transplantation , receptor
. To assess the effects of cyclosporin (CyA) on endothelin‐1 (ET‐1) in rat liver allograft rejection, we evaluated ET‐1 expression in samples obtained from BN(RTl n )‐to‐BN (group 1) rats, DA(RTl a )‐to‐BN (group 2) rats, and DA‐to‐BN rats treated with 5 mg/kg per day of CyA (group 3). Serum and hepatic ET‐1 levels, determined by a radioimmunoassay, remained unchanged in group 1. In group 2, the ET‐1 levels peaked on postoperative day (POD) 5 in the liver at 344 ± 31.6 pg/ g wet, and on POD 7 in the serum at 38.7 ± 13.1 pg/ml. In group 3, hepatic and renal ET‐1 levels showed a progressive increase until POD 10, while serum ET‐1 levels remained unchanged. In conclusion, acute rejection caused a temporary increase in the ET‐1 level in both the serum and the liver in the early postoperative period what might have been caused by endothelial damage due to ongoing, acute rejection. CyA caused a time‐dependent increase in the ET‐1 level in both the liver and the kidney without an increase in the serum ET‐1 level. The serum ET‐1 level might have been affected by the clearance of ET from the liver or kidney.