Open AccessEvidence for the presence of ferritin in plant mitochondria
Author(s) -
Zancani Marco,
Peresson Carlo,
Biroccio Antonino,
Federici Giorgio,
Urbani Andrea,
Murgia Irene,
Soave Carlo,
Micali Fulvio,
Vianello Angelo,
Macrì Francesco
Publication year - 2004
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.2004.04300.x
Subject(s) - ferritin , mitochondrion , immunoprecipitation , arabidopsis , biochemistry , immunocytochemistry , biology , arabidopsis thaliana , fragmentation (computing) , etiolation , chemistry , microbiology and biotechnology , mutant , gene , enzyme , ecology , endocrinology
In this work, evidence for the presence of ferritins in plant mitochondria is supplied. Mitochondria were isolated from etiolated pea stems and Arabidopsis thaliana cell cultures. The proteins were separated by SDS/PAGE. A protein, with an apparent molecular mass of approximately 25–26 kDa (corresponding to that of ferritin), was cross‐reacted with an antibody raised against pea seed ferritin. The mitochondrial ferritin from pea stems was also purified by immunoprecipitation. The purified protein was analyzed by MALDI‐TOF mass spectrometry and the results of both mass finger print and peptide fragmentation by post source decay assign the polypeptide sequence to the pea ferritin ( P < 0.05). The mitochondrial localization of ferritin was also confirmed by immunocytochemistry experiments on isolated mitochondria and cross‐sections of pea stem cells. The possible role of ferritin in oxidative stress of plant mitochondria is discussed.