Open AccessStructural and Serological Characterization of the O‐Antigenic Polysaccharide of the Lipopolysaccharide from Acinetobacter Strain 90 Belonging to DNA Group 10
Author(s) -
Haseley Simon R.,
Holst Otto,
Brade Helmut
Publication year - 1997
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1997.t01-1-00470.x
Subject(s) - polysaccharide , chemistry , sephadex , galactose , biochemistry , chromatography , monosaccharide , antiserum , hydrolysis , polyclonal antibodies , enzyme , antigen , biology , genetics
Water‐soluble lipopolysaccharide (phenol/water extraction) isolated from Acinetobacter strain 90, which belongs to DNA group 10, was hydrolysed with 1 % acetic acid, ultracentrifuged, and water‐soluble products finally eluted from a Sephadex G‐50 column. The major fraction, a polysaccharide, contained D‐Gal, D‐GlcNAc, D‐GalNAc, and 4,6‐dideoxy‐4‐[( R )‐3‐hydroxybutyramido]‐D‐galactose (Fuc4NBuOH). The polysaccharide was characterised by means of monosaccharide analyses, Smith‐degradation, N‐deacetylation/deamination, and NMR studies, and was shown to have a branched pentasaccharide repeating unit.This structure was specifically recognized in western blots and enzyme immunoassays by polyclonal rabbit antisera.