Open AccessDeletion Analysis of the Schistosoma Mansoni 28‐kDa Glutathione S ‐transferase Gene Promoter in Mammalian Cells
Author(s) -
Serra Esteban,
Zemzoumi Khalid,
Dissous Colette
Publication year - 1997
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1997.t01-1-00113.x
Subject(s) - microbiology and biotechnology , schistosoma mansoni , activator (genetics) , luciferase , biology , glutathione s transferase , gene , reporter gene , transfection , promoter , glutathione , biochemistry , gene expression , enzyme , schistosomiasis , helminths , zoology
The 1241‐bp promoter region of the Schistosoma mansoni 28‐kDa glutathione S ‐transferase gene (Sm28GST) was sequentially deleted and analyzed using the luciferase reporter gene system in different cell lines. The activator protein‐1 (AP‐1) site located at –231 seems to be responsible for the major part of the promoter activity. The 1241‐bp Sm28GST promoter was not, in transient transfection experiments, activated by reagents generating reactive oxygen species, such as hydrogen peroxide (H 2 O 2 ), 3‐methyl‐