Open AccessStructural Studies of the Cell‐Envelope Oligosaccharide from the Lipopolysaccharide of Haemophilus Influenzae Strain RM.118‐28
Author(s) -
Risberg Anna,
Schweda Elke K. H.,
Jansson PerErik
Publication year - 1997
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1997.00701.x
Subject(s) - heptose , chemistry , oligosaccharide , haemophilus influenzae , phosphocholine , lipid a , mass spectrometry , electrospray ionization , nuclear magnetic resonance spectroscopy , lipopolysaccharide , anhydrous , hydrolysis , chromatography , stereochemistry , biochemistry , organic chemistry , phosphatidylcholine , phospholipid , mutant , biology , membrane , gene , endocrinology , antibiotics
The structure of the oligosaccharide part of the Haemophilus influenzae RM.118‐28 lipopolysaccharide (LPS) has been investigated. The oligosaccharide was obtained from the LPS by mild acid hydrolysis followed by gel‐permeation chromatography, and was studied by methylation analysis, NMR spectroscopy and mass spectrometry. The structure of the major compound, which is a hexasaccharide, is proposed as follows.In the structure, Kdo is 3–deoxy‐ d ‐ manno ‐octulosonic acid, PE tn is phosphoethanolamine, P Cho is phosphocholine and l,d ‐Hep is l ‐ glycero ‐ d ‐ manno ‐heptose. Electrospray‐ionization mass spectrometry on O‐deacylated LPS obtained after treatment with anhydrous hydrazine gave evidence for the presence of two minor compounds, which show additional substitution of the main structure with phosphate and P Etn, respectively. These substitutions have not been localized.