Open AccessWidespread Tissue Expression of Gastrin‐Binding‐Protein Mrna
Author(s) -
Monstein HansJürg,
Nylander AnnaGreta,
Håkanson Rolf
Publication year - 1997
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1997.00502.x
Subject(s) - gastrin , messenger rna , complementary dna , microbiology and biotechnology , northern blot , binding protein , biology , peptide sequence , autocrine signalling , biochemistry , receptor , gene , secretion
Glycine‐extended forms of gastrin (gastrin‐Gly) are thought to be involved in the autocrine growth control of colorectal carcinomas. The recently described gastrin‐binding protein has been suggested to be a gastrin‐Gly accepting receptor. Northern blot analysis demonstrated the expression of gastrin‐binding‐protein mRNA in many tissues of mouse, rat, and man. The gastrin‐binding‐protein mRNA expression was confirmed by reverse‐transcribed PCR analysis. Analysis of the cDNA and the deduced ammo acid sequence of the PCR‐amplified rat gastrin‐binding‐protein DNA fragments revealed sequence identity (except in a single position) with the corresponding human and pig gastrin‐binding protein and with the a‐subunit of a rat and human mitochondrial trifunctional enzyme, involved in fatty acid oxidation. The widespread and abundant tissue expression of gastrin‐binding‐protein mRNA and its sequence identity with a fatty‐acid‐oxidizing enzyme do not support the view that it represents a genuine gastrin receptor.