Open AccessElucidation of the Structure of the Core Region and the Complete Structure of the R‐Type Lipopolysaccharide of Erwinia carotovora FERM P‐7576
Author(s) -
Fukuoka Satoshi,
Knirel Yuriy A.,
Lindner Buko,
Moll Hermann,
Seydel Ulrich,
Zähringer Ulrich
Publication year - 1997
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1997.00055.x
Subject(s) - chemistry , mass spectrometry , heptose , erwinia , nuclear magnetic resonance spectroscopy , stereochemistry , moiety , cysteine , lipopolysaccharide , biochemistry , chromatography , mutant , biology , gene , enzyme , endocrinology
An R‐type lipopolysaccharide (LPS) from Erwinia carotovora strain FERM P‐7576 was studied after strong alkaline degradation and mild acid hydrolysis. The resulting products were analyzed by fast‐atom bombardment mass spectrometry, one‐ and two‐dimensional 1 H and 13 C NMR spectroscopy, dephosphorylation and methylation analysis. The following structure was proposed for the core region of the LPS:where Hep is l ‐ glycero ‐ d ‐ manno ‐heptose and Kdo is 3‐deoxy‐ d ‐ manno ‐octulosonic acid. Some LPS species lack the β‐ d ‐Gal p residue or the β‐ d ‐Gal p ‐(1→7)‐α‐Hep p disaccharide. With the known structures of lipid A [Fukuoka, S., Kamishima, H., Nagawa, Y., Nakanishi, H., Ishikawa, K., Niwa, Y., Tamiya, E. & Karube, I. (1992) Arch. Microbiol. 157 , 311–318] and the core moiety, the complete LPS structure was established and confirmed by matrix‐assisted laser desorption/ionisation time‐of‐flight mass spectrometry of the native and O‐deacylated LPS.