Open AccessGene Structure of the Murine Calcium Channel β3 Subunit, cDNA and Characterization of Alternative Splicing and Transcription Products
Author(s) -
Murakami Manabu,
Wissenbach Ulrich,
Flockerzi Veil
Publication year - 1996
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1996.t01-1-00138.x
Subject(s) - exon , alternative splicing , intron , scn3a , gene , biology , calcium channel , genetics , protein subunit , complementary dna , coding region , n type calcium channel , microbiology and biotechnology , rna splicing , protein primary structure , peptide sequence , g alpha subunit , rna , t type calcium channel , calcium , chemistry , organic chemistry
The β3 subunit of high‐voltage‐gated calcium channels is a peripheral membrane protein that copurifies with neural N‐type calcium channels. Murine genomic clones containing the full coding sequence of β3 were isolated and the exons were mapped and sequenced. The murine calcium channel β3 subunit is encoded by a unique gene composed of 13 translated exons that spread over ≈8 kb of genomic sequence. Alternatively spliced transcripts of the β3 gene were identified and characterized. The primary structure of β3 is highly conserved between the murine, human, rabbit and rat proteins (98% identity). The intron placement within that primary structure correlates with the previously postulated exon positions in transcripts encoding the members of the calcium channel β subunit family and confirm a close evolutionary relationship of the β3, β1, β2 and β4 subunit genes.