Open AccessTransient‐State Kinetic Evidence Against a Channelled Transfer of Glyceraldehyde 3‐Phosphate from Aldolase to Glyceraldehyde‐3‐Phosphate Dehydrogenase
Author(s) -
Arias Wilma Martínez,
Pettersson Gösta
Publication year - 1996
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1996.0675u.x
Subject(s) - aldolase a , glyceraldehyde 3 phosphate dehydrogenase , glyceraldehyde , dehydrogenase , aldolase b , kinetics , fructose bisphosphate aldolase , chemistry , enzyme , biochemistry , kinetic energy , phosphate , physics , quantum mechanics
Theoretical and experimental evidence is presented which leads to a rejection of the proposal that the transient‐state kinetics of the coupled two‐enzyme reaction involving aldolase and glyceraldehyde‐3‐phosphate dehydrogenase are indicative of a channelled transfer of glyceraldehyde 3‐phosphate from the producing enzyme to the consuming one.