Open AccessA TGACG Motif Mediates Growth‐Hormone‐Factor‐1/Pituitary‐Transcriptional‐Activator‐1‐Dependent cAMP Regulation of the Rainbow Trout Growth‐Hormone Promoter
Author(s) -
Argenton Francesco,
Bernardini Serena,
Puttini Stefania,
Colombo Lorenzo,
Bortolussi Marino
Publication year - 1996
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1996.0591w.x
Subject(s) - biology , microbiology and biotechnology , transcription factor , activator (genetics) , anterior pituitary , effector , gene , promoter , luciferase , trout , transfection , gene expression , hormone , endocrinology , genetics , fishery , fish <actinopterygii>
The mechanisms involved in the regulation of the rainbow trout growth hormone ( tGH ) gene promoter by the pituitary‐specific transcription factor GHF1 (growth hormone factor 1), also called Pit1 (pituitary transcriptional activator 1), and cAMP have been investigated in mammalian and fish cells. The ‐340 to +24 5′‐flanking region of the t GH gene fused to the luciferase gene was activated in rat pituitary GC cells and in HeLa cells cotransfected with an effector plasmid encoding rat GHF1. GC cell nuclear extracts produced four GHF1‐specific footprints (sites F1 to F4) on the t GH promoter, each containing multiple W 4 NCAT (W, A or T) or closely related motifs. Mutational analysis performed in GC cells indicated that the proximal F1 site alone can direct transcription, but that the region encompassing the F2 and F3 sites is necessary for optimal activation and contains a TGACG motif (cAMP‐response element, CRE) confering cAMP responsiveness. The role of the TGACG motif in mediating cAMP regulation of the t GH promoter was confirmed in primary cultures of trout pituitary cells. Cotransfection studies in carp EPC cells using an effector plasmid encoding trout GHF1 demonstrated the GHF1 dependence of cAMP stimulation. Gel shift and southwestern experiments revealed nuclear proteins of 43 kDa and 30 kDa in GC and fish cells, respectively, that bind specifically to the t GH CRE, suggesting the involvement of CRE‐binding‐protein/activating‐transcription‐factor‐1‐related peptides in cAMP response. Incidentally, and in contrast with previous reports, we found the rat GH promoter, that lacks TGACG motifs, unresponsive to cAMP. Thus, the cAMP stimulation of the t GH gene is more similar to its human counterpart, that is also GHF1 dependent and mediated by TGACG motifs in the promoter. It is suggested that control of GH gene expression has evolved modularly, through various assortments of the same regulatory units, rather than molecularly, through innovative units.