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Activation of Signaling Pathways in HL60 Cells and Human Neutrophils by Farnesylthiosalicylate
Author(s) -
Tisch Daphna,
Halpern Matya,
Marciano Daniela,
Kloog Yoel,
Aviram Irit
Publication year - 1996
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1996.0529r.x
Subject(s) - nadph oxidase , pertussis toxin , superoxide , protein kinase c , microbiology and biotechnology , phospholipase c , cytosol , hl60 , intracellular , inositol trisphosphate , second messenger system , biology , signal transduction , chemistry , inositol , biochemistry , receptor , in vitro , g protein , enzyme , reactive oxygen species
Effects of the farnesylcysteine mimetic, farnesylthiosalicylate on the activation of myeloid cells were studied. In dimethyl‐sulfoxide‐differentiated HL60 cells and in human neutrophils farnesylthiosalicylate (≤20 μM) dose‐dependently elevated cytosolic Ca 2+ concentrations, suggesting phospholipase‐C‐mediated release of the ion from intracellular stores. In human neutrophils, in addition to the production of inositol trisphosphate, farnesylthiosalicylate induced activation of the NADPH oxidase and translocation of the cytosolic oxidase components p47‐phox and p67‐phox to the membrane. The calcium signal, inositol‐trisphosphate production and superoxide generation elicited by farnesylthiosalicylate were partially blocked by treatment of the cells with pertussis toxin, consistent with participation of pertussis‐toxin‐sensitive and pertussis‐toxin‐resistant elements. In HL60 cells, farnesylthiosalicylate (≤20 μM) did not activate NADPH oxidase but dose‐dependently augmented PMA‐elicited activity of the enzyme. This effect was resistant to pertussis‐toxin treatment. In vitro augmentation of PKC‐mediated phosphorylation of histone and cytosolic p47‐phox by farnesylthiosalicylate and the finding that downregulation of PKC abrogated potentiation of NADPH oxidase activity by farnesylthiosalicylate were compatible with the involvement of PKC in the response of HL60 cells to farnesylthiosalicylate. It is suggested that the effects of farnesylthiosalicylate on myeloid cells reflect interaction of the analog with prenylcysteine‐docking sites on cellular signaling elements.

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