Open AccessThe O‐Specific Polysaccharide Chain of Campylobacter fetus Serotype B Lipopolysaccharide is a d ‐Rhamnan Terminated with 3‐ O ‐Methyl‐ d ‐Rhamnose ( d ‐Acofriose)
Author(s) -
Senchenkova Sof'ya N.,
Shashkov Alexander S.,
Knirel Yuriy A.,
Mcgovern Joseph J.,
Moran Anthony P.
Publication year - 1996
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1996.0434u.x
Subject(s) - polysaccharide , chemistry , rhamnose , nuclear magnetic resonance spectroscopy , oligosaccharide , methyl iodide , residue (chemistry) , methylation , hydrolysis , stereochemistry , two dimensional nuclear magnetic resonance spectroscopy , disaccharide , chromatography , biochemistry , organic chemistry , gene
An O‐specific polysaccharide was liberated from Campylobacter fetus subsp. fetus serotype B lipopolysaccharide by mild acid hydrolysis followed by gel chromatography. This polysaccharide was found to contain d ‐rhamnose and 3‐ O ‐methyl‐ d ‐rhamnose ( d ‐Rha3Me, d ‐acofriose) in a ratio of approximately 24:1, as well as lipopolysaccharide core constituents. The structure of the polysaccharide was studied by 1 H‐NMR and 13 C‐NMR spectroscopy, which included two‐dimensional COSY, rotating‐frame NOE spectroscopy (ROESY), and computer‐assisted analysis of the 13 C‐NMR spectrum. Methylation analysis using [ 2 H 3 ]methyl iodide and Smith degradation followed by GLC/MS of the derived acetylated oligosaccharide‐alditols was used to determine the location of d ‐acofriose. The O‐specific polysaccharide is linear, consists on average of 12 disaccharide repeating units, and is terminated by a residue of d ‐acofriose. The following structure of the d ‐rhamnan chain was established: