Open AccessStructural Studies of the Putative O‐Specific Polysaccharide of Acinetobacter baumannii O11
Author(s) -
Haseley Simon R.,
Wilkinson Stephen G.
Publication year - 1996
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1996.0266n.x
Subject(s) - galactose , polysaccharide , acinetobacter baumannii , polymer , chemistry , hydrolysis , aqueous solution , moiety , strain (injury) , biochemistry , stereochemistry , organic chemistry , bacteria , biology , anatomy , pseudomonas aeruginosa , genetics
A major polysaccharide containing d ‐galactose, d ‐glucose and 2‐acetamido‐2‐deoxy‐ d ‐galactose was obtained after mild acid hydrolysis of the water‐soluble material released by treatment of cell walls from Acinetobacter baumannii strain Oil with hot, aqueous phenol. By means of NMR studies, Smith degradation and N‐deacetylation/deamination, the repeating unit of the polymer was identified as a branched pentasaccharide of the structure shown. Also present was a minor polymer containing glucose, 2‐aceta‐mido‐2‐deoxyglucose and 2‐acetamido‐2‐deoxygalactose, the structure of which was not elucidated. On serological testing, the polymeric material was shown to correspond to the O‐antigenic moiety of the parent extract (assumed to be lipopolysaccharide) and circumstantial evidence indicated that Oil specificity was conferred by the major polymer.