Open AccessCloning, Sequencing and Sites of Expression of Genes for the Hydroxyarginine‐Containing Adhesive‐Plaque Protein of the Mussel Mytilus galloprovincialis
Author(s) -
Inoue Koji,
Takeuchi Yasuhiro,
Miki Daisuke,
Odo Satoshi,
Harayama Shigeaki,
Waite J. Herbert
Publication year - 1996
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1996.0172u.x
Subject(s) - complementary dna , biology , gene , untranslated region , microbiology and biotechnology , cloning (programming) , nucleic acid sequence , coding region , genetics , rapid amplification of cdna ends , molecular cloning , rna , computer science , programming language
A segment of Mytilus galloprovincialis foot protein 3 (Mgfp‐3) cDNA was amplified by means of reverse‐transcription (RT)/PCR with degenerate primers. The 5′ and 3′ regions of the cloned segment were amplified by means of rapid amplification of cDNA ends. The 5′‐region clones had almost identical nucleotide sequences, but two sequences were found among 3′‐region clones. The Mgfp‐3 coding region was amplified between a 5′ untranslated sequence and one of two 3′ untranslated sequences. Two cDNA clones which encoded variants Mgfp‐3A and Mgfp‐3B, were isolated. These two clones encoded proteins with 70 and 77 amino acid residues, of which the first 24 residues are predicted to be signal peptides. The existence of additional variants was suggested by the sequences of other clones. Thus, it was suggested that Mgfp‐3 genes constitute a gene family. RTPCR of RNA from developing larvae indicates that Mgfp‐3 genes are transcribed after settlement. RT/PCR of RNA from major organs and in situ hybridization of the foot indicate that Mgfp‐3 genes are transcribed in a limited part of the foot, i.e., the phenol gland and a distal part of the accessory gland.