Open AccessCloning, Sequencing and Expression Analysis of Mouse Cartilage Matrix Protein cDNA
Author(s) -
Aszódi Attila,
Hauser Nik,
Studer Daniel,
Paulsson Mats,
Hiripi László,
Bósze Zsuzsa
Publication year - 1996
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1996.00970.x
Subject(s) - complementary dna , microbiology and biotechnology , messenger rna , in situ hybridization , rapid amplification of cdna ends , biology , cloning (programming) , reverse transcriptase , open reading frame , cartilage , reverse transcription polymerase chain reaction , molecular cloning , peptide sequence , polymerase chain reaction , gene , anatomy , genetics , computer science , programming language
A cDNA encoding the mouse cartilage matrix protein (CMP) was cloned following the reverse‐transcription polymerase chain reaction and rapid amplification of cDNA ends procedures using mRNA isolated from trachea. The open reading frame encodes a product of 500 amino acids. Large parts of the protein have been completely conserved when compared to chicken and human sequences, including all 12 cysteine residues of the mature CMP. In situ hybridization reveals an even distribution of the CMP mRNA in the developing skeleton, which is followed by a zonal distribution paralelling hypertrophy and calcification. From early cartilage differentiation and onwards, CMP transcript is absent in the forming articular surfaces and intervertebral discs. Extraskeletal expression of CMP mRNA was detected in the adult eye.