Open Access1 H‐NMR Signal of Arenicola marina Myoglobin in vivo as an Index of Tissue Oxygenation
Author(s) -
Kreutzer Ulrike,
Jue Thomas
Publication year - 1996
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1996.00622.x
Subject(s) - in vivo , arenicola , oxygenation , myoglobin , chemistry , heme , intertidal zone , proton nmr , hemeprotein , intracellular , biology , biochemistry , biophysics , stereochemistry , enzyme , ecology , genetics
Key questions on how intertidal animals adapt to hypoxic stress center on the high energy phosphate response to decreasing oxygenation. With recent 1 HP/ 31 P‐NMR techniques to monitor mammalian tissue metabolism, a novel approach has emerged to observe potentially the intracellular oxygen interaction in invertebrates. The present study indicates that Arenicola marina , a standard model for intertidal animals, exhibits a distinct set of Mb 1 H‐NMR signals in vivo , corresponding to the two isolated Mb isoforms. Specitically both deoxy‐Mb I and deoxy‐Mb IT exhibit paramagnetically shifted signals at 93.4 ppm and 92.5 ppm at 25 °C, respectively, which arise from the proximal histidyl N δ H. These signals reflect the cellular oxygenation state and indicate clearly that the phasphotautocyamine level begins to drop at the onset of anoxia and declines gradually to 50% of control after 3.5 h. 1 H Mb spectra indicate protein heterogeneity originating from heme as well as structural disorder.