Open AccessThe Incorporation of Arachidonic Acid into Triacylglycerol in P388D 1 Macrophage‐Like Cells
Author(s) -
Balsinde Jesús,
Dennis Edward A.
Publication year - 1996
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1996.00480.x
Subject(s) - diacylglycerol kinase , monoacylglycerol lipase , arachidonic acid , phospholipid , biochemistry , fatty acid , phosphatidate , chemistry , metabolism , enzyme , protein kinase c , endocannabinoid system , receptor , membrane
When P388D 1 cells are incubated in media containing 10 μM [ 3 H] aragudibuc acid ( 4 , Ach), the tabel is rapidly incorporated into phospholipids and triacylglycerol. However, incorporation of [ 3 H] 4 , Ach into phospholipids clearly precedes incorporation into triacylglycerol, indicating that the phospholipid pool constitutes the primary metabolic fate of the 4 Ach via a remodelling pathway. In contrast, [ 3 H] 4 , Ach is incorporated into triacylglycerol almost exclusively via de novo synthesis, as evidenced by studies using propranolol, a phosphatidate phosphohydrolase inhibitor. This compound induced a time‐dependent and concentration‐dependent increase in the levels of [ 3 H] 4 Ach‐containing phosphatidate that is directly correlated with a dccreasc in the levels or [ 3 H] 4 , Ach‐containing triacylglycerol. In addition, no change in the levels of [ 3 H] 4 , Ach‐containing diacylglycerol and monoacylglycerol were apparent along the time course of fatty acid incorporation into triacylglycerol. However, a sharp and transient accumulation of cell‐associated free [ 3 H] 4 Ach wits detected shortly after exposure of the cells to the radioactive fatty acid. Collectively, the results reported herein suggest that free 4 , Ach availability determines the patterns of incorporation and distribution of this fatty acid among the various lipid classes of P388D, cells.