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Conserved Alternative Splicing in the 5′‐Untranslated Region of the Muscle‐Specific Enolase Gene
Author(s) -
Oliva Daniele,
Venturella Silvana,
Passantino Rosa,
Feo Salvatore,
Giallongo Agata
Publication year - 1995
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1995.tb20792.x
Subject(s) - biology , alternative splicing , five prime untranslated region , untranslated region , polysome , rna splicing , gene isoform , complementary dna , microbiology and biotechnology , messenger rna , gene , three prime untranslated region , genetics , ribosome , rna
We report here the isolation and characterization of cDNAs covering the 5′‐end region of mouse and rat mRNAs that encode the β or muscle‐specific isoform of the glycolytic enzyme enolase. As previously determined for humans, two classes of β‐enolase transcripts with distinct sequences in their 5′‐untranslated regions are present in both mouse and rat muscles. A mechanism of alternative splicing, conserved from mouse to man, generates the two forms of mRNA. Secondary‐structure predictions indicated that, in all cases, a more stable secondary structure could exist in the 5′ end of the message with the longer leader. In vitro transcripts containing defined human or mouse 5′‐untranslated sequences were obtained by fusion of the different cDNA clones and tested for their relative translational efficiencies in rabbit reticulocyte lysates. Transcripts containing the human long and short leader sequences showed differences in the translational rate, suggesting a role for the 5′‐untranslated region in the regulation of translation. No detectable difference was found between transcripts with the two distinct mouse leader sequences. In addition, both transcripts are bound to polysomes and are equally distributed along differently sized polysomes in C2C12 myogenic cells. The relative expression of the two spliced forms in developing and adult muscle tissues by means of reverse transcription and polymerase chain reaction did not show a stage‐specific or a tissue‐type‐specific pattern. A putative functional role for the 5′‐untranslated sequences of β‐enolase transcripts is discussed.

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