Open AccessBacterial Expression of the Catalytic Domain of 3–Hydroxy‐3–Methylglutaryl‐CoA Reductase (Isoform HMGR1) from Arabidopsis thaliana , and Its Inactivation by Phosphorylation at Ser577 by Brassica oleracea 3‐Hydroxy‐3‐Methylglutaryl‐CoA Reductase Kinase
Author(s) -
Dale Susan,
Arró Montserrat,
Becerra Beatriz,
Morrice Nick G.,
Boronat Albert,
Hardie D. Grahame,
Ferrer Albert
Publication year - 1995
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1995.506_2.x
Subject(s) - arabidopsis thaliana , reductase , biochemistry , arabidopsis , phosphorylation , kinase , protein kinase a , biology , enzyme , mutant , gene
The catalytic domain of 3–hydroxy‐3–methylglutaryl‐CoA reductase isoform 1 (HMGR1cd) from Arabidopsis thaliana has been expressed in Escherichia coli in a catalytically active form and purified. The high efficiency of the bacterial expression system together with the simplicity of the purification procedure used in this study resulted in the attainment of large quantities of pure enzyme (about 5 mg/l culture) with a final specific activity of up to 17 U/mg. This specific activity is higher than that reported to date for any 3–hydroxy‐3–methylglutaryl‐CoA reductase (HMGR) purified from a plant source. HMGR1cd activity was completely blocked by the HMGR inhibitor mevinolin (IC 50 = 12.5nM). No significant differences were observed between the K m values of HMGR1cd for NADPH (71 ± 7 μM) and ( S )‐3–hydroxy‐3–methylglutaryl‐CoA (8.3 ± 1.5 μM) and those of pure HMGR preparations obtained from different plant sources. The purified HMGR1cd was reversibly inactivated by phosphorylation at a single site by Brassica oleracea HMGR kinase A, which is functionally related to the mammalian AMP‐activated protein kinase. The site of phosphorylation is Ser577 in the complete sequence of A. thaliana HMGR1. The results in this paper represent the first evidence that a higher plant HMGR is regulated by direct phosphorylation, at least in a cell‐free system. Our results also reinforce the view that the AMP‐activated protein kinase/SNF1 family is an ancient and highly conserved protein kinase system.