Open AccessValyl‐tRNA Synthetase from Artemia
Author(s) -
Brandsma Margreet,
Kerjan Pierre,
Dijk Jan,
Janssen George M. C.,
Möller Wim
Publication year - 1995
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1995.277_1.x
Subject(s) - brine shrimp , artemia salina , biology , transfer rna , biochemistry , yeast , elongation factor , eukaryotic translation elongation factor 1 alpha 1 , microbiology and biotechnology , rna , chemistry , gene , ribosome , fishery , organic chemistry , toxicity
Two components of the protein biosynthetic machinery, valyl‐transfer RNA synthetase (VRS) and elongation factor 1 (EF‐1), have been isolated as a complex from several mammalian tissues. However, yeast VRS, which lacks an amino‐terminal extension, does not associate with EF‐1. We purified VRS from the brine shrimp Artemia and investigated its interaction with EF‐1. Western blotting of crude Artemia extracts revealed the presence of two forms of VRS, differing in size and capacity to associate with EF‐1. About 80% of the total VRS corresponds to a polypeptide of 130 kDa which‐behaves as a monomer upon gel filtration. Only the larger form of 140 kDa coelutes, cosediments and co‐immunoprecipitates with the EF‐1α 2 βγδ complex. The ratio of the two forms of VRS remains constant throughout early development. The possible origin and mode of expression of the two forms of VRS present in Anemia are discussed.