Open Access3‐Deoxy‐Octulosonic‐Acid‐Containing Hexasaccharide Fragment of Unusual Core Type Isolated from Hafnia alvei 2 Lipopolysaccharide
Author(s) -
Ravenscroft Neil,
Gamian Andrzej,
Romanowska Elżbieta
Publication year - 1995
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1995.0889p.x
Subject(s) - microbiology and biotechnology , lipopolysaccharide , hafnia , fragment (logic) , chemistry , biology , immunology , organic chemistry , cubic zirconia , ceramic , computer science , programming language
The hexasaccharide containing 3‐deoxy‐octulosonic acid (Kdo) was isolated from the carbohydrate material obtained after the mild acid hydrolysis of lipopolysaccharide of Hafnia alvei strain 2. The hexasaccharide was purified by gel filtration on Bio‐Gel P‐4 and P‐2 columns. On the basis of sugar and methylation analyses, one‐ and two‐dimensional NMR spectroscopic methods, the hexasaccharide was identified asThe tetrasaccharide linked to position 7 of Kdo is also part of the sialic‐acid‐containing O‐specific unit. Di‐substitution of Kdo at positions 7 and 8 has not been previously reported.