Open AccessMolecular Cloning and Nuclear Localization of ATBP, A Novel (A+T)‐Stretch‐binding Protein of Sarcophaga peregrina (Flesh Fly)
Author(s) -
NakanishiMatsui Mayumi,
Kubo Takeo,
Natori Shunji
Publication year - 1995
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1995.0396h.x
Subject(s) - zinc finger , complementary dna , microbiology and biotechnology , biology , intron , enhancer , gene , binding protein , molecular cloning , transcription factor , dna binding protein , biochemistry
Previously, we purified a DNA‐binding protein, named ATBP, from cultured Sarcophaga cells. This protein was found to bind specifically to (A+T) stretches that locate in the 5′‐upstream region and the intron of the Sarcophaga lectin gene [Matsui, M., Kobayashi, A., Kubo, T. & Natori, S. (1994) Eur. J. Biochem. 219 , 449–454]. Here, we report molecular cloning of a cDNA for ATBP. The cDNA encoded a protein with two zinc‐finger motifs, an acidic domain, two glutamine‐rich domains and a glutamine run. Immunofluorescence study revealed that ATBP was localized in the nuclei. Thus, ATBP is possibly a transcription factor for genes having (A+T) stretches in their promoter and/or enhancer regions.