Open AccessIsolation and complete sequence of CBR , a gene encoding a putative cytochrome b reductase in Saccharomyces cerevisiae
Author(s) -
CSUKAI Michael,
MURRAY Michael,
ORR Elisha
Publication year - 1994
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1994.tb19957.x
Subject(s) - reductase , biochemistry , saccharomyces cerevisiae , biology , cytochrome p450 reductase , cytochrome b , gene , yeast , nucleic acid sequence , 7 dehydrocholesterol reductase , flavoprotein , cytochrome b5 , cytochrome , genetics , enzyme , mitochondrial dna
We have isolated and characterised a novel yeast gene, CBR (cytochrome b reductase), encoding a 35‐kDa yeast novobiocin‐binding protein. The predicted protein sequence of CBR displays considerable similarity to both plant nitrate reductases and mammalian cytochrome b 5 reductases indicating that it is a putative member of the flavoprotein pyridine‐nucleotide‐cytochrome‐reductase family. Disruption of CBR is not lethal under various growth conditions, suggesting the presence of some functional overlap with other reductases, possibly with the cytochrome P ‐450 reductase.