Open AccessThe structure of the O‐specific chain of Legionella pneumophila serogroup 1 lipopolysaccharide
Author(s) -
KNIREL Yuriy A.,
RIETSCHEL Ernst Th.,
MARRE Reinhard,
ZÄHRINGER Ulrich
Publication year - 1994
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1994.tb18734.x
Subject(s) - lipopolysaccharide , legionella pneumophila , monosaccharide , strain (injury) , microbiology and biotechnology , chemistry , polysaccharide , legionella , bacteria , stereochemistry , biochemistry , biology , genetics , immunology , anatomy
The O‐polysaccharide chain of Legionella pneumophila Philadelphia strain 1 (serogroup 1) lipopolysaccharide was investigated by means of 1 H‐ and 13 C‐NMR spectroscopy, and chemical analysis. It was found to consist of an α‐(2→4) interlinked homopolymer of a 5‐acetamidino‐7‐acetamido‐8‐ O ‐acetyl‐3,5,7,9‐tetradeoxy‐nonulosonic acid possessing most likely the d ‐ glycero ‐ l ‐ galacto configuration, representing the first example of an acidic homopolymer of a higher sugar of this class. The ladder‐like banding pattern exhibiting small distances between individual bands in the SDS/PAGE is compatible with a monosaccharide repeating unit.