Open AccessThe protective role of high‐density lipoprotein on oxidized‐low‐density‐lipoprotein‐induced U937/endothelial cell interactions
Author(s) -
MAIER Jeanette Anne Marie,
BARENGHI Livia,
PAGANI Franco,
BRADAMANTE Silvia,
COMI Paola,
RAGNOTTI Giovanni
Publication year - 1994
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1994.tb18712.x
Subject(s) - low density lipoprotein receptor related protein 8 , high density lipoprotein , low density lipoprotein , lipoprotein , u937 cell , chemistry , biochemistry , cholesterol , very low density lipoprotein , apoptosis
The adherence of monocytes to the endothelium is an early event in atherogenesis. We have investigated this process by examining whether native and oxidized low‐density and high‐density lipoproteins could modulate this process. Only oxidized low‐density lipoprotein caused a significant dose‐dependent and time‐dependent increase in U937 monocyte‐like cell line binding to human endothelial cells, by a process which required de novo protein synthesis. Interstingly, E‐selectin, intercellular adhesion molecule‐1, vascular cell‐adhesion molecule or P‐selectin induction was not apparent in this system suggesting the presence of an alternative system for the interaction of endothelial cells with monocyte‐like cells in response to oxidized low‐density lipoprotein. Highdensity lipoprotein completely suppressed oxidized low‐density‐lipoprotein‐induced adhesion of U937 cells to the endothelial monolayer, while oxidized high‐density lipoprotein did not. These data suggest that the balance between native and oxidized lipoproteins may play a role in the formation of the atherosclerotic lesion by modulating monocyte endothelial interactions.