Open AccessAnalysis of genes encoding highly conserved lysine‐rich proteins in Aplysia californica and Saccharomyces cerevisiae
Author(s) -
AUCLAIR Daniel,
LANG B. Franz,
FOREST Pierre,
DESGROSEILLERS Luc
Publication year - 1994
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1994.tb18704.x
Subject(s) - complementary dna , biology , gene , saccharomyces cerevisiae , gene expression , southern blot , microbiology and biotechnology , cdna library , blot , northern blot , yeast , genetics
To isolate a gene that can be used as an internal control in studies on gene expression in Aplysia californica neurons, we have characterized a cDNA clone (pKRP‐A) isolated on the basis of its high expression in A. californica neurons. This cDNA is of 850 nucleotides and codes for a putative 29‐kDa lysine‐rich protein. Blotting experiments revealed that the gene is expressed in all tested A. californica tissues, and in individually identified neurons of the abdominal ganglion, suggesting that this gene can be efficiently used as internal control in studies of gene expression. We have also isolated one cDNA and two different genomic clones from yeast libraries that show 59% identity with pKRP‐A. Sequence comparison of genomic clones, as well as PCR and Southern blotting experiments, revealed that at least two homologous genes are present in yeast. Northern blotting experiments revealed that the expression of the gene is strongly repressed at 39°C.