Open AccessStructural Phase Transitions Involved in the Interaction of Phospholipid Bilayers with Octyl Glucoside
Author(s) -
Maza Alfonso,
Parra Jose Luis
Publication year - 1994
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1994.t01-1-01029.x
Subject(s) - vesicle , pulmonary surfactant , bilayer , octyl glucoside , chemistry , liposome , dynamic light scattering , phospholipid , phosphatidylcholine , lipid bilayer , aqueous solution , chromatography , critical micelle concentration , partition coefficient , analytical chemistry (journal) , micelle , crystallography , membrane , chemical engineering , organic chemistry , biochemistry , nanoparticle , engineering
The transitional stages induced by the interaction of the nonionic surfactant octyl glucoside (OcOse) on phosphatidylcholine liposomes were studied by means of transmission electron microscopy (TEM), light scattering and permeability changes. A linear correlation was observed between the effective surfactant/lipid molar ratio ( Re three‐stage model proposed for liposome solubilization) and the OcOse concentration in the initial and final interaction stages, despite showing almost a constant value during bilayer saturation. The bilayer/aqueous phase partition coefficient ( K ) decreased in the subsolubilizing interaction steps and increased during solubilization. Thus, whereas a preferential distribution of surfactant monomers in the aqueous phase with respect to the lipid bilayers took place in the initial interaction steps, a larger association of OcOse molecules with these lipids in bilayers occured during solubilization. The initial steps of bilayer saturation (50–70% permeability) were attained for a lower free surfactant (S w ) than that for its critical micellar concentration (cmc). When S w reached the OcOse cmc, solubilization started to occur ( Re sat ). Large unilamellar vesicles began to form as the OcOse exceeded 60 mol/100 mol, exhibiting for 65 mol/100 mol (50% permeability) vesicles of approximately 400 nm. TEM pictures for 100% permeability (72 mol/100 mol) and Re sat still showed unilamellar vesicles, albeit that the Re sat TEM picture showing traces of smaller structures. Exceeding surfactant amounts led to a decrease in static light scattering; the vesicle‐size curve began to show a bimodal distribution. The TEM picture showed tubular structures together with bilayer fragments. Thereafter, the open structures were gradually affected by the surfactant and the scattered intensity gradually decreased to a constant low value.