Open AccessIsolation and 1 H‐NMR Spectroscopic Identification of Poly(3‐Hydroxybutanoate) from Prokaryotic and Eukaryotic Organisms
Author(s) -
Seebach Dieter,
Brunner Andreas,
Bürger H. Michael,
Schneider Jakob,
Reusch Rosetta N.
Publication year - 1994
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1994.00317.x
Subject(s) - isolation (microbiology) , identification (biology) , chemistry , stereochemistry , biology , microbiology and biotechnology , botany
Trace amounts of poly[( R )‐3‐hydroxybutanoate] were isolated from competent Escherichia coli , spinach, bovine serum albumin, beef heart mitochondria, and aortal tissues, all sources in which it is not accumulated as storage material. Its identity was in all cases proved by 1 H‐NMR spectroscopy. In some runs, the poly[( R )‐3‐hydroxybutanoate] isolated from competent E. coli also contained some 3‐hydroxyvalerate, an observation confirmed by 1 H‐NMR spectroscopy and gas chromatography. The absolute configuration of the polymers isolated from E. coli and spinach was shown to be (all‐ R ) by gas chromatography on chiral columns.