Open AccessTranscriptional induction of β‐galactoside α‐2,6‐sialyltransferase in rat fibroblast by dexamethasone
Author(s) -
VANDAMME Valérie,
PIERCE Annick,
VERBERT André,
DELANNOY Philippe
Publication year - 1993
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1993.tb19879.x
Subject(s) - sialyltransferase , galactoside , dexamethasone , glucocorticoid , messenger rna , fibroblast , biology , chemistry , glycoprotein , biochemistry , medicine , endocrinology , microbiology and biotechnology , gene , enzyme , in vitro
The β‐galactoside α‐2,6‐sialyltransferase activity of Fisher rat fibroblasts is enhanced by dexamethasone while the activity of the β‐galactoside α‐2,3‐sialyltransferase remains unchanged. This glucocorticoid‐dependent activation can be inhibited by the antagonist RU 38.486 and results from an elevated transcription rate of the 4.7‐kb mRNA previously characterized in rat fibroblasts, distinct from the 4.3‐kb liver‐restricted mRNA. As shown by the binding of radiolabelled Sambucus nigra agglutinin, this activation leads to an increase of NeuNAc(α2–6)Gal sequences on glycoproteins isolated from the dexamethasone‐treated cells.