Open AccessThe expression of human plasma cholesteryl‐ester‐transfer protein in HepG2 cells in induced by sodium butyrate
Author(s) -
SPERKER Bernhard,
MARK Michael,
BUDZINSKI RalphM.
Publication year - 1993
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1993.tb18451.x
Subject(s) - cholesterylester transfer protein , sodium butyrate , chemistry , messenger rna , butyrate , cell culture , human plasma , biochemistry , microbiology and biotechnology , biology , chromatography , gene , cholesterol , lipoprotein , genetics , fermentation
Although human plasma cholesteryl‐ester‐transfer protein (CETP) is primarily synthesized in the liver, its expression in a number of transformed liver cell lines is very low. However the use of the human hepatoma cell line HepG2 as a model system for the regulation of CETP on mRNA level is facilitated by a quantitative reverse‐transcribed polymerase chain reaction. We demonstrate a time‐dependent and concentration‐dependent 3–4‐fold induction of CETP mRNA by sodium butyrate. CETP mass in the medium is also augmented; however, the effect on protein level is less pronounced.