Open AccessOverexpression of Desulfovibrio vulgaris Hildenborough cytochrome c 553 in Desulfovibrio desulfuricans G200
Author(s) -
BLANCHARD Laurence,
MARION Dominique,
POLLOCK Brent,
VOORDOUW Gerrit,
WALL Judy,
BRUSCHI Mireille,
GUERLESQUIN Françoise
Publication year - 1993
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1993.tb18377.x
Subject(s) - desulfovibrio vulgaris , desulfovibrio , chemistry , biochemistry , cytochrome , bacteria , biology , genetics , enzyme
Plasmid pRC41, containing the cyf gene encoding cytochrome c 553 from Desulfovibrio vulgaris Hildenborough, was transferred by conjugation from Escherichia coli to Desulfovibrio desulfuricans G200. The structural properties of the purified protein were studied by one‐dimensional and two‐dimensional NMR. A heterogeneity in the folding of the cytochrome isolated from D. vulgaris Hildenborough and from D. desulfuricans G200 was observed for the oxidized form. Temperature, pH and salt‐dependence studies indicated that the heterogeneity does not result from an intermediate in the protein unfolding process, but derives from two conformations which are not in dynamic equilibrium.