Open AccessStructural requirements for processing of pro‐adipokinetic hormone I
Author(s) -
RAYNE Richard C.,
O'SHEA Michael
Publication year - 1993
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1993.tb18320.x
Subject(s) - residue (chemistry) , loop (graph theory) , chemistry , side chain , stereochemistry , biochemistry , mathematics , organic chemistry , combinatorics , polymer
We found that a seven‐residue sequence in pro‐adipokinetic hormone I (proAKH I) which precedes the endopeptidase cleavage site is predicted to form an Ω loop. Molecular modelling experiments indicated that a stable Ω loop may form at this site, and suggested that loop stability may depend on the C‐terminal loop residue, Lys12. The importance of this residue in proAKH I processing was confirmed by the observation that replacement of Lys12 by thialysine, a Lys analog with an altered side chain, prevented processing in vivo. In addition we showed by molecular modelling that this side‐chain alteration may prevent formation of an Ω loop. Together, these approaches lead us to propose that an Ω loop may serve as a recognition motif in proAKH I processing.