Open AccessInteractions of calcium and magnesium with the mitochondrial inorganic pyrophosphatase from Saccharomyces cerevisiae
Author(s) -
URIBE Salvador,
RANGEL Pablo,
PARDO Juan Pablo,
PEREIRADASILVA Lucia
Publication year - 1993
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1993.tb18289.x
Subject(s) - inorganic pyrophosphatase , pyrophosphatase , pyrophosphate , saccharomyces cerevisiae , calcium , magnesium , chemistry , mitochondrion , biochemistry , yeast , enzyme , organic chemistry
The activity of the mitochondrial inorganic pyrophosphatase from Saccharomyces cerevisiae was measured in the presence of increasing concentrations of magnesium and calcium. Calcium pyrophosphate (dissociation constant K d = 1.9 μM) inhibited pyrophosphatase by competition with magnesium pyrophosphate ( K d = 50 μM). The small movements of calcium detected in mitochondria from yeast may be physiologically significant for the control of inorganic pyrophosphatase activity and the concentration of pyrophosphate in the matrix of yeast mitochondria.