Open AccessStructural study of a cell‐wall mannan of Saccharomyces kluyveri IFO 1685 strain
Author(s) -
SHIBATA Nobuyuki,
KOJIMA Chizuko,
SATOH Yohko,
SATOH Richi,
SUZUKI Atsuko,
KOBAYASHI Hidemitsu,
SUZUKI Shigeo
Publication year - 1993
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1993.tb18211.x
Subject(s) - mannan , mannose , heteronuclear molecule , homonuclear molecule , two dimensional nuclear magnetic resonance spectroscopy , nuclear magnetic resonance spectroscopy , chemistry , saccharomyces cerevisiae , strain (injury) , stereochemistry , yeast , polysaccharide , biochemistry , biology , organic chemistry , molecule , anatomy
Acetolysis of the cell‐wall mannan of Saccharomyces kluyveri under mild conditions, gave fragments with 1–6 mannose residues. The structures of mannopentaose and mannohexaose were determined to beandrespectively, by two‐dimensional homonuclear Hartmann‐Hahn spectroscopy and a sequential NMR assignment method that combines 1 H‐ 13 C correlated spectroscopy, relayed coherence transfer spectroscopy, 1 H‐detected heteronuclear multiple‐bond connectivity and methylation analysis. The H1 proton chemical shift of a neighboring α‐1,2‐linked mannose unit of the 3‐ O ‐substituted structure was shifted upfield by the addition of a mannose unit to the adjacent 3 O ‐substituted unit by an α‐1,6 linkage. The characteristic H1–H2‐correlated cross‐peak of the α‐1,3‐linked mannose unit substituted by a β‐1,2 linkage, β1→2Manα1→3, in the mannan of S. kluyveri , as also found by two‐dimensional homonuclear Hartmann‐Hahn spectroscopy in the mannan of Candida guilliermondii , a pathogenic yeast in man.