Open AccesscDNA cloning of the sodium‐influx‐stimulating peptide in the mollusc, Lymnaea stagnalis
Author(s) -
SMIT August B.,
THIJSEN Steven F. T.,
GERAERTS Wijnand P. M.
Publication year - 1993
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1993.tb18046.x
Subject(s) - lymnaea stagnalis , cloning (programming) , complementary dna , lymnaea , peptide , microbiology and biotechnology , biology , chemistry , biochemistry , snail , ecology , gene , computer science , programming language
We isolated and characterized a cDNA clone, encoding the prohormone of the sodium‐influxstimulating (SIS) peptide of the freshwater snail Lymnaea stagnalis . The prohormone is cleaved to generate a signal peptide of 23 amino acids and a SIS peptide of 77 amino acids. The SIS peptide as encoded by the cDNA represents a novel and complex neuropetide, which controls the activity of sodium pumps in the integument, pericardium, ureter and nephridial gland. In situ hybridization showed that the SIS‐peptide gene is expressed by the neuroendocrine, so‐called Yellow Cells of the central nervous system.