Open AccessChemoattractants for neutrophils in lipopolysaccharide‐induced inflammatory exudate from rats are not interleukin‐8 counterparts but gro‐gene‐product/melanoma‐growth‐stimulating‐activity‐related factors
Author(s) -
WATANABE Kazuyoshi,
IIDA Megumi,
TAKAISHI Kiyosumi,
SUZUKI Tadaaki,
HAMADA Yoko,
IIZUKA Yoshio,
TSURUFUJI Susumu
Publication year - 1993
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1111/j.1432-1033.1993.tb17920.x
Subject(s) - chemotaxis , lipopolysaccharide , sephadex , exudate , cytokine , chemistry , interleukin , microbiology and biotechnology , biology , biochemistry , immunology , receptor , enzyme , botany
Potent chemotactic activity for neutrophils was detected in rat inflammatory exudate induced by a subcutaneous injection of lipopolysaccharide in a carboxymethyl‐cellulose suspension. We purified and characterized chemoattractants from the exudate by the following procedures: carboxymethyl Sephadex C‐25 ion‐exchange chromatography; G3000SW gel‐filtration chromatography; preparative reverse‐phase high‐pressure liquid chromatography; rechromatography on reverse‐phase HPLC. Two chemotactic factors were purified and their N‐terminal amino acid sequences were determined. One factor was a protein in which the first 20 N‐terminal amino acids were identical to those of rat cytokine‐induced neutrophil chemoattractant (CINC), a counterpart of human gro/melanoma growthstimulating activity (MGSA). The other factor was highly similar to mouse macrophage inflammatory protein 2 (MIP‐2). Mouse MIP‐2, a chemotactic factor for neutrophils, is a member of the interleukin‐8 family; however the protein we purified had higher similarity to human gro/MGSA than to human interleukin‐8. These results indicate that, in rats, chemotactic factors for neutrophils induced by lipopólysaccharide stimulation are not counterparts of interleukin‐8, but are gro/CINC‐related peptides.